Influenza 2009 pandemic H1N1 A/Hamburg/5/09_100HA

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Experiment Name	Influenza 2009 pandemic H1N1 A/Hamburg/5/09_100HA
Associated Publication	, ,
Analyte Name	A/Hamburg/5/09, H1N1 virus
Analyte Family	Viruses/Virus-like particles
Analyte Information	Beta-propiolactone inactivated pandemic A(H1N1) 2009 Influenza Virus isolated from a clinical specimen (a case of mild infection), propagated in MDCK cells and passaged solely in these cells. Haemagglutinin 222D.
Concentration	100 HA units
Protocol	After blocking arrayed slides with 3% (w/v) bovine serum albumin (Sigma A8577), 0.02% Blocker Casein (Pierce) in 150mM sodium chloride and 5 mM calcium chloride, the suspension of the inactivated virus (100 HA titre) was assayed in the presence of 17.5 uM oseltamivir carboxylate (19C for 1 h). After fixation with 4% paraformaldehyde, bound viruses were detected using the rabbit anti-A/New Jersey/76 antiserum (1:200), followed by biotinylated Protein A (Sigma), 10 ug/ml, and Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	Please note that the HA titre was measured under a different assay set up compared to that in publication PMID19741625. 2000 HA here equals to 100 HA in the abovementioned paper.