Anti-betα-glucan antibody mAb 2G8-IgG2_2015 data

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Experiment Name	Anti-betα-glucan antibody mAb 2G8-IgG2_2015 data
Associated Publication	Unravelling glucan recognition systems by glycome microarrays using the designer approach and mass spectrometry. Palma AS, Liu Y, Zhang H, Zhang Y, McCleary BV, Yu G, Huang Q, Guidolin LS, Ciocchini AE, Torosantucci A, Wang D, Carvalho AL, Fontes CM, Mulloy B, Childs RA, Feizi T, Chai W Molecular & cellular proteomics : MCP, 14, 974-88 2015 Apr
Analyte Name	mAb 2G8-IgG2b
Analyte Family	Antibodies
Analyte Information	Murine hybridoma IgG2b antibody, produced by Balb/c mouse immunization with a glyco-conjugate vaccine containing a soluble Candida albicans beta-glucan preparation (GGZym) conjugated to the recombinant, genetically inactivated diphtheria toxin CRM197. mAb 2G8-IgG2b was shown to target several fungal pathogens, such as Candida albicans, Aspergillus fumigatus and Cryptococcus neoformans and to provide protection in multiple models of fungal diseases, including vaginal and systemic Candida infections and invasive aspergillosis or cryptococcosis
Concentration	0.1 ug/ml
Protocol	After blocking arrayed slides with 0.02% (v/v) Blocker Casein (Pierce), 1% (w/v) bovine serum albumin (Sigma A8577) in Hepes buffered saline (5 mM Hepes, pH 7.4, 150 mM NaCl, 5 mM CaCl2), mAb 2G8-IgG2b was assayed at 0.1 ug/ml, followed by biotinylated anti-mouse IgG (Sigma) at 10 ug/ml. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	N/A