y-ArtinM

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Experiment Name	y-ArtinM
Associated Publication	Glycan microarray analysis of the carbohydrate-recognition specificity of native and recombinant forms of the lectin ArtinM. Liu Y, Cecílio NT, Carvalho FC, Roque-Barreira MC, Feizi T Data in brief, 5, 1035-47 2015 Dec
Analyte Name	ArtinM (yeast)
Analyte Family	Plant lectins
Analyte Information	y-ArtinM was recombinantly expressed in Saccharomyces cerevisiae BJ3501 and obtained by yeast lysis. It was purified by affinity chromatography on a D-mannose column, ultradiafiltered against PBS and biotinylated using sulfo-NHS-LC-biotin. ArtinM is a D-mannose-binding lectin that exerts immunomodulatory and regenerative activities through its carbohydrate recognition domain.
Concentration	50 ug/ml
Protocol	After blocking arrayed slides with Blocker Casein (Pierce) containing 1% (w/v) bovine serum albumin (Sigma A8577), biotinylated y-ArtinM was assayed at 50 ug/ml. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	N/A