Influenza 2009 pandemic H1N1 A/Hamburg/5/09 (D222G)_200HA

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Experiment Name	Influenza 2009 pandemic H1N1 A/Hamburg/5/09 (D222G)_200HA
Associated Publication	Altered receptor specificity and cell tropism of D222G hemagglutinin mutants isolated from fatal cases of pandemic A(H1N1) 2009 influenza virus. Liu Y, Childs RA, Matrosovich T, Wharton S, Palma AS, Chai W, Daniels R, Gregory V, Uhlendorff J, Kiso M, Klenk HD, Hay A, Feizi T, Matrosovich M Journal of virology, 84, 12069-74 2010 Nov
Analyte Name	A/Hamburg/5/09-E (222G), H1N1 virus
Analyte Family	Viruses/Virus-like particles
Analyte Information	Beta-propiolactone inactivated viruse derived from A/Hamburg/5/09 by egg passage and plaque purification in MDCK cells and differed by a single substitution, haemagglutinin D222G.
Concentration	100 HA units
Protocol	After blocking arrayed slides with 3% (w/v) bovine serum albumin (Sigma A8577), 0.02% Blocker Casein (Pierce) in 150mM sodium chloride and 5 mM calcium chloride, the suspension of the inactivated virus (100 HA titre) was assayed in the presence of 17.5 uM oseltamivir carboxylate (19C for 1 h). After fixation with 4% paraformaldehyde, bound viruses were detected using the rabbit anti-A/New Jersey/76 antiserum (1:200), followed by biotinylated Protein A (Sigma), 10 ug/ml, and Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	Please note that the HA titre was measured under a different assay set up compared to that in publication PMID19741625. 2000 HA here equals to 100 HA in the abovementioned paper.