B-Lymphotropic Polyomavirus capsid viral protein 1 (LPyV VP1)

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Experiment Name	B-Lymphotropic Polyomavirus capsid viral protein 1 (LPyV VP1)
Associated Publication	Structures of B-lymphotropic polyomavirus VP1 in complex with oligosaccharide ligands. Neu U, Khan ZM, Schuch B, Palma AS, Liu Y, Pawlita M, Feizi T, Stehle T PLoS pathogens, 9, e1003714 2013 Oct
Analyte Name	LpyV VP1 capsid
Analyte Family	Viral proteins
Analyte Information	Amino acids 28-301 of LPyV VP1 fused to a N-terminal hexa-histidine tag and recombinantly expressed in E. coli. VP1 is the major viral capsid protein and is responsible for virus attachment to host cells and initiation of infection.
Concentration	150 ug/ml
Protocol	After blocking arrayed slides with 3% (w/v) bovine serum albumin (Sigma A8577) in Hepes buffered saline (5 mM Hepes, pH 7.4, 150 mM NaCl, 5 mM CaCl2), JCV-VP1 was assayed precomplexed with mouse monoclonal anti-poly-histidine and biotinylated anti-mouse IgG antibodies (both from Sigma) in a ratio of 4:2:1 (by weight) and overlaid onto the arrays at 150 ug/ml. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	NA