Seasonal influenza H1N1 A/Memphis/14/96-M

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Experiment Name	Seasonal influenza H1N1 A/Memphis/14/96-M
Associated Publication	Receptor-binding specificity of pandemic influenza A (H1N1) 2009 virus determined by carbohydrate microarray. Childs RA, Palma AS, Wharton S, Matrosovich T, Liu Y, Chai W, Campanero-Rhodes MA, Zhang Y, Eickmann M, Kiso M, Hay A, Matrosovich M, Feizi T Nature biotechnology, 27, 797-9 2009 Sep
Analyte Name	A/Memphis/14/96-M, H1N1 virus
Analyte Family	Viruses/Virus-like particles
Analyte Information	Beta-propiolactone inactivated seasonal H1N1 virus propagated in MDCK cells and passaged solely in these cells.
Concentration	400 HA units
Protocol	After blocking arrayed slides with 3% (w/v) bovine serum albumin (Sigma A8577), 0.02% Blocker Casein (Pierce) in 150mM sodium chloride and 5 mM calcium chloride, the suspension of the inactivated virus (400 HA titre) was assayed in the presence of 17.5 uM oseltamivir carboxylate. After fixation with 4% paraformaldehyde, bound viruses were detected using a 1:1:1 anti-sera mix of rabbit anti-A/New Jersey/76, anti-A/Egypt/96/02(H1N2) and anti-Brazil/11/78(H1N1) (1:200), followed by biotinylated Protein A (Sigma), 10 ug/ml, and Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	In this publication suspensions of the viruses were adjusted to contain equivalent concentrations of HA glycoprotein as assessed by gel electrophoresis; we refer to these as standard concentrations. This experiment was at virus standard concentration. Ple