Micronemal protein 3 of Eimeria tenella, tandem MAR domains 1α-1b-1d-1e (EtMIC3-MAR5)

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Experiment Name	Micronemal protein 3 of Eimeria tenella, tandem MAR domains 1α-1b-1d-1e (EtMIC3-MAR5)
Associated Publication	The role of sialyl glycan recognition in host tissue tropism of the avian parasite Eimeria tenella. Lai L, Bumstead J, Liu Y, Garnett J, Campanero-Rhodes MA, Blake DP, Palma AS, Chai W, Ferguson DJ, Simpson P, Feizi T, Tomley FM, Matthews S PLoS pathogens, 7, e1002296 2011 Oct
Analyte Name	Eimeria tenella MIC3-MAR5
Analyte Family	Other microbial proteins
Analyte Information	EtMIC3-MAR5 (comprising sequences encompassing MAR1a, MAR1b, MAR1d and MAR1e residues 1-298,750-921) fused to a N-terminal hexahistidine-thioredoxin tag, and recombinantly expressed in E. coli.
Concentration	40 ug/ml
Protocol	After blocking arrayed slides with 1% (w/v) bovine serum albumin (Sigma A8577) in Blocker Casein (Pierce), EtMIC3- MAR5 was overlaid at 40 ug/ml, and followed by overlay with anti-polyhistidine and biotinylated anti-mouse IgG antibodies (10 ug/ml, precomplexed in a ratio of 1:1). Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	NA