Micronemal protein 4 of Toxoplasma gondii , APPLE domain 5 (TgMIC4-A5)

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Experiment Name	Micronemal protein 4 of Toxoplasma gondii , APPLE domain 5 (TgMIC4-A5)
Associated Publication	Galactose recognition by the apicomplexan parasite Toxoplasma gondii. Marchant J, Cowper B, Liu Y, Lai L, Pinzan C, Marq JB, Friedrich N, Sawmynaden K, Liew L, Chai W, Childs RA, Saouros S, Simpson P, Roque Barreira MC, Feizi T, Soldati-Favre D, Matthews S The Journal of biological chemistry, 287, 16720-33 2012 May 11
Analyte Name	T. gondii MIC4-A5
Analyte Family	Other microbial proteins
Analyte Information	TgMIC4-A5 comprising amino acid residues 410-491 of TgMIC4 fused to a N-terminal hexahistidine-thioredoxin tag, and recombinantly expressed in E. coli.
Concentration	20 ug/ml
Protocol	After blocking arrayed slides with 1% (w/v) bovine serum albumin (Sigma A8577) in Blocker Casein (Pierce), TgMIC4-A5 was precomplexed with mouse monoclonal anti-poly-histidine and biotinylated anti-mouse IgG antibodies (both from Sigma) in a ratio of 1:2.5:2.5 (by weight) and overlaid onto the arrays at 20 ug/ml. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	NA