anti-prostate cancer antibody F77_small array, 0.1 ug/ml (37 degrees)

Instructions | Symbol Definitions | Lipid Tag Descriptions
(Asterisks that follow the names of certain probes indicate that predominant components are shown.)

Sort By Value
Display Errors

Textual Symbolic

Experiment Name	anti-prostate cancer antibody F77_small array, 0.1 ug/ml (37 degrees)
Associated Publication	Carbohydrate sequence of the prostate cancer-associated antigen F77 assigned by a mucin O-glycome designer array. Gao C, Liu Y, Zhang H, Zhang Y, Fukuda MN, Palma AS, Kozak RP, Childs RA, Nonaka M, Li Z, Siegel DL, Hanfland P, Peehl DM, Chai W, Greene MI, Feizi T The Journal of biological chemistry, 289, 16462-77 2014 Jun 6
Analyte Name	F77
Analyte Family	Antibodies
Analyte Information	Monoclonal antibody F77 is an murine IgG3 antibody, raised against human prostate cancer cell line PC3.
Concentration	0.1 ug/ml
Protocol	After blocking the arrayed slides with 3% w/v BSA in Hepes-buffered-saline (5 mM Hepes, pH 7.4, 150 mM NaCl, 5 mM CaCl2), mAb F77 IgG3 was overlaid at 0.1 ug/ml,at 37 C followed by biotinylated anti-mouse IgG(Sigma) at 1:200. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml). As diluent the blocker solution was used.
Other Comments	NA