Broadly neutralizing anti-HIV monoclonal antibody 3BC315

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Experiment Name	Broadly neutralizing anti-HIV monoclonal antibody 3BC315
Associated Publication	Broad neutralization by a combination of antibodies recognizing the CD4 binding site and a new conformational epitope on the HIV-1 envelope protein. Klein F, Gaebler C, Mouquet H, Sather DN, Lehmann C, Scheid JF, Kraft Z, Liu Y, Pietzsch J, Hurley A, Poignard P, Feizi T, Morris L, Walker BD, Fätkenheuer G, Seaman MS, Stamatatos L, Nussenzweig MC The Journal of experimental medicine, 209, 1469-79 2012 Jul 30
Analyte Name	3BC315
Analyte Family	Antibodies
Analyte Information	One of the human HIV-1 neutralizing IgG antibodies isolated using a cell-based capture method. It has been shown to bind to a broadly neutralizing epitope in the vicinity of the V3 loop and the CD4-induced site and highly complement the neutralization spectrum of potent broadly neutralizing anti-CD4 binding site (CD4bs) antibodies obtained from the same individual.
Concentration	10 ug/mL
Protocol	After blocking arrayed slides with 0.04% (v/v) Blocker Casein (Pierce), 1% (w/v) bovine serum albumin (Sigma A8577) in Hepes buffered saline (5 mM Hepes, pH 7.4, 150 mM NaCl, 5 mM CaCl2), 3BC315 was precomplexed with biotinylated anti-human IgG (Vector) at a 1:3 ratio (w/w) before application onto the slides at a final concentration of 10 ug/ml. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml).
Other Comments	N/A