Broadly neutralizing anti-HIV monoclonal antibody 2G12_2013 data

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Experiment Name	Broadly neutralizing anti-HIV monoclonal antibody 2G12_2013 data
Associated Publication	Broad neutralization by a combination of antibodies recognizing the CD4 binding site and a new conformational epitope on the HIV-1 envelope protein. Klein F, Gaebler C, Mouquet H, Sather DN, Lehmann C, Scheid JF, Kraft Z, Liu Y, Pietzsch J, Hurley A, Poignard P, Feizi T, Morris L, Walker BD, Fätkenheuer G, Seaman MS, Stamatatos L, Nussenzweig MC The Journal of experimental medicine, 209, 1469-79 2012 Jul 30
Analyte Name	2G12
Analyte Family	Antibodies
Analyte Information	Broadly neutralizing human monoclonal IgG antibody against HIV-1 that has an epitope centred on the high-mannose and/or hybrid N-glycan residues on the viral spike glycoprotein gp120.
Concentration	10 ug/mL
Protocol	After blocking arrayed slides with 0.04% (v/v) Blocker Casein (Pierce), 1% (w/v) bovine serum albumin (Sigma A8577) in Hepes buffered saline (5 mM Hepes, pH 7.4, 150 mM NaCl, 5 mM CaCl2), 2G12 was precomplexed with biotinylated anti-human IgG (Vector) at a 1:3 ratio (w/w) before application onto the slides at a final concentration of 10 ug/ml. Binding was detected using Alexa Fluor-647-labeled streptavidin from Molecular Probes (1 ug/ml).
Other Comments	N/A